Biochemical Analysis of Chromatin Containing RecombinantDrosophila Core Histones
نویسندگان
چکیده
منابع مشابه
In vitro transcription of chromatin containing histones hyperacetylated in vivo.
The culture of cells in the presence of sodium n-butyrate causes an accumulation of histones that are highly acetylated. When chromatin containing these histones was transcribed with E. coli RNA polymerase, an increase in the template activity compared to control chromatin was observed. Titration of chromatin with polymerase under both reinitiating and non-reinitiating conditions showed there w...
متن کاملIsolation of four core histones from human sperm chromatin representing a minor subset of somatic histones.
Using high performance liquid chromatography we have successfully purified four core histones from mature human sperm chromatin. The H2A variants present in sperm (H2A.X and limited H2A.Z) have been shown previously to be minor variants in somatic chromatin. The histones are highly modified as evidenced by extensive acetylation and an as yet uncharacterized multicharge modification of H2B. Base...
متن کاملProteins: histones and chromatin.
Patrick Cramer is a professor of Biochemistry at the Ludwig Maximilians University of Munich, Germany, and head of the Gene Center Munich. He is a structural biologist who has contributed to our understanding of the structure and function of RNAP II from yeast and its associated factors. In a recent study, his laboratory described the structure of RNAP II in complex with the initiation factor T...
متن کاملChromatin: Hanging on to histones
The core histones have long been known to have an important role in the packaging of DNA into nucleosomes. Today, nucleosome assembly is recognized as an integral component of both transcriptional activation and repression [1]. Transcriptional control is mediated through the assembly of extensive nucleoprotein structures containing both conventional transcription factors and architectural facto...
متن کاملChromatin, Histones, and Epigenetic Tags
POPULÄRVETENSKAPLIG SAMMANFATTNING LIST OF PUBLICATIONS LIST OF ABBREVIATIONS INTRODUCTION 15 Chromatin History and Research 16 Nobel Prizes Related to Chromatin 19 AIMS OF THE STUDY 21 CHROMATIN STRUCTURE 23 DNA 23 Composition of DNA 23 The Nucleosome 26 Location of Linker Histones 27 Higher-Order Chromatin Structure 28 Euchromatin and Heterochromatin 30 THE HISTONES 31 Histone Variants 32 Cor...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2002
ISSN: 0021-9258
DOI: 10.1074/jbc.m111212200